Figure 5

Immunohistochemical analysis of orexin-A expression in the lateral hypothalamus. Region framed in red on the brain picture indicates the site of histological recordings. The mean integrated signal density was calculated from sections from 3–4 mice per group. Female animals on normal diet (ND) are shown in (A), males in (B). Female animals on high fat diet (HFD) are shown in (C), males in (D). The scale bar represents 250 µm. ***p < 0.001 difference between the genotypes having the same feeding schedule ⁺⁺⁺p < 0.001 difference between the feeding schedule within the same genotypes, each using Šidákś test.