Figure 2
From: Selective Targeting of Virus Replication by Proton Pump Inhibitors
Flavi- and Picornaviruses exhibited tenatoprazole resistance; MAYV exhibited susceptibility. 293 T cells were pre-treated for 6 hr (DENV, ZIKV, MAYV) or 7 hr (PV) with media containing either DMSO (0) or the indicated concentration of tenatoprazole. Cells were then infected with Panel A, the synthetic DENV 2 strain 16681; panel B, ZIKV; Panel C, the Mahoney strain of PV at MOI = 0.01; Panel D, MAYV at MOI ~0.001. After two hours, the tissue culture media was removed and replaced with fresh treatment media. After two days, the media was collected and the virus titer was measured by focus forming assay where foci were developed with 4g2 primary anti-flavivirus antibody (DENV: the y axis shows the relative change compared to the DMSO control) or plaque assay in Vero cells (ZIKV, PV, MAYV). Plaques were visualized by staining with crystal violet. The y-axis show the virus titers in plaque-forming units. Error bars equal 1 SD.
