Figure 1

Changes in chickens with SX9 infection. (A) Experimental design, including viral inoculation time and sample collection time. (B) Viral load in different chicken tissues quantified by RT-qPCR (nā=ā6). (C) Histopathological changes in the ilea of chickens challenged with the NIBV strain SX9 [H&E staining]. The black arrow shows the shed villus epithelial tissue, and the red arrow shows the goblet cells (vacuole). The villus height (D) and crypt depth (E) and the ratio of the villus height to the crypt depth (V/C) (F) of the ilea were measured by Image-Pro Plus 6.0. The data are shown as the means, with SEMs in (D to F). The asterisk superscripts on the bars indicate significant differences compared with the control group (t test, *Pā<ā0.05).