Figure 3 | Scientific Reports

Figure 3

From: Anxiolytic Drug FGIN-1-27 Ameliorates Autoimmunity by Metabolic Reprogramming of Pathogenic Th17 Cells

Figure 3

Effect of FGIN-1-27 on Th17 Differentiation is Independent of TSPO. (a) Plot showing correlation between binding of FGIN-1-27 to TSPO and effect on IL-17 production. Binding of FGIN-1-27 to TSPO was measured using a biochemical radiolabeled displacement assay and radiolabeled PK-11195 was used as a control ligand. (b) Immunoblot to detect TSPO from spleens of either control or TSPO knockout mice (upper panel) and dose response plot for FGIN-1-27 showing effect on Th17 differentiation using purified CD4 T cells from spleens of either control or TSPO knockout mice. IL-17 production was normalized using DMSO treatment from control cells as 100%. Data are representative of 3 independent experiments and the error bars represent SD.

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