Table 2 Evaluation of the extraction recovery, matrix effect, and precision in the UPLC-MS/MS analysis of standard mixtures of DSV, TAM, and TOH with OND (IS).
| Â | Concentration spiked (ng/ml) | Extraction recovery | Matrix effect | Precision | |||||
|---|---|---|---|---|---|---|---|---|---|
Intra-day (n = 6) | Inter-day (n = 18) | ||||||||
Mean recovery (%) ± RSDa | Er(%)b | Mean recovery (%) ±RSDa | Er(%)b | Mean recovery (%) ±RSDa | Er(%)b | Mean recovery (%) ±RSDa | Er(%)b | ||
DSV | 20 | 96.37 ± 1.66 | −3.63 | 97.78 ± 2.32 | −2.22 | 98.60 ± 1.19 | −1.40 | 97.43 ± 2.06 | −2.57 |
60 | 97.05 ± 1.59 | −2.95 | 97.28 ± 2.45 | −2.72 | 97.22 ± 1.92 | −2.78 | 98.65 ± 1.89 | −1.35 | |
500 | 96.30 ± 1.47 | −3.70 | 93.65 ± 4.56 | −6.35 | 98.21 ± 1.95 | −1.79 | 98.12 ± 0.66 | −1.88 | |
800 | 90.11 ± 6.38 | −9.89 | 99.32 ± 1.85 | −0.68 | 97.67 ± 2.17 | −2.33 | 97.77 ± 1.03 | −2.23 | |
TAM | 0.1 | 95.05 ± 2.51 | −4.95 | 96.21 ± 1.32 | −3.79 | 95.58 ± 3.28 | −4.42 | 93.70 ± 2.24 | −6.30 |
0.3 | 97.40 ± 2.47 | −2.60 | 96.66 ± 1.83 | −3.34 | 98.57 ± 0.79 | 1.43 | 97.89 ± 1.52 | −2.11 | |
100 | 96.48 ± 1.12 | −3.52 | 92.26 ± 3.01 | −7.74 | 99.07 ± 3.98 | −0.93 | 99.13 ± 0.76 | −0.87 | |
400 | 89.01 ± 7.88 | −10.99 | 98.89 ± 7.54 | −1.11 | 98.47 ± 2.44 | −1.53 | 98.77 ± 1.75 | −1.23 | |
TOH | 0.5 | 101.68 ± 2.33 | 1.68 | 97.96 ± 0.99 | −2.04 | 99.95 ± 3.04 | −0.05 | 98.08 ± 1.43 | −1.92 |
1.5 | 95.82 ± 5.65 | −4.18 | 97.73 ± 1.28 | −2.27 | 99.41 ± 2.04 | −0.59 | 99.65 ± 1.74 | −0.35 | |
150 | 99.78 ± 0.99 | −0.22 | 99.02 ± 2.44 | −0.98 | 96.36 ± 3.05 | −3.64 | 98.80 ± 1.57 | −1.20 | |
400 | 91.95 ± 3.21 | −8.05 | 101.45 ± 2.78 | 1.45 | 98.01 ± 2.02 | −1.99 | 97.25 ± 2.61 | −2.75 | |
