Table 4 Evaluation of the stability of DSV, TAM, and TOH in rat plasma.

From: Development and validation of UPLC-MS/MS method for studying the pharmacokinetic interaction of dasabuvir and tamoxifen, 4-hydroxytamoxifen in Wistar rats

Stability

Concentration level*

Mean recovery (%) ± RSDa

DSV

TAM

TOH

Auto-sampler stability

(10 °C, 56 h)

Low

95.46 ± 1.77

98.25 ± 2.56

99.67±1.35

 

High

98.49 ± 0.72

93.46 ± 2.43

95.15 ± 5.66

Short-term stability

(25 °C, 6 h)

Low

96.35 ± 3.32

98.22 ± 1.21

96.88 ± 2.77

 

High

95.05 ± 2.68

96.77 ± 2.85

98.90 ± 0.52

Long-term stability

(−30 °C, 30 days)

Low

96.82 ± 3.62

99.69 ± 1.04

99.03 ± 3.19

 

High

98.82 ± 2.60

97.30 ± 4.59

95.82 ± 1.22

Freeze-thaw stability

(−30 °C, 3 cycles)

Low

98.51 ± 0.49

96.11 ± 3.21

97.49 ± 2.09

 

High

98.54 ± 1.59

93.93 ± 6.05

97.59 ± 0.63

Refrigerator (4 °C, 3 months)

Low

96.23 ± 3.54

98.91 ± 2.09

97.99 ± 5.06

 

High

97.57 ± 2.10

96.61 ± 2.84

97.03 ± 0.95

  1. *Two concentration levels were evaluated for each analyte; low (200, 1, 5 ng/mL for DSV, TAM, and TOH, respectively) and high (800, 400, 400 ng/mL for DSV, TAM, and TOH, respectively).
  2. aMean recovery (%) ± RSD of six determinations.
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