Figure 6

Integrated metabolic pathway enrichment analysis. (a) Gene-metabolite joint pathways were identified using the MetaboAnalyst integrated pathway analysis module. (b) Gene-metabolite network exploration identified a GOT1- and GOT2-mediated interactive network that might influence the TCA cycle and other metabolic pathways. (c–e) Box plots of GOT1 expression in malignant prostate tumor compared to normal (data sources https://www.oncomine.org/resource/login.html). (f) A bar graph of GOT1 expression across the prostate cancer Gleason score (GS) (data sources GSE30521). (g–i) A volcano plots of the differential metabolites (g), bar graph of glutamate level among normal, tumor, and metastatic clinical specimens of Pan-Cancer Metabolism Data Explorer (h), and ROC curve for glutamate level prediction in PCa urine samples compared to normal (i). Statistical significance was analyzed by Student’s t-test.