Table 1 Differential phenotypic characteristics of strain HM134T and the type strains of related species Strains: Strains: 1, HM134T; 2, M. rifamycinica AM105T; 3, M. wenchangensis CCTCC AA 2012002 T; 4, M. mangrovi 2803GPT1-18T.

From: Micromonospora zhangzhouensis sp. nov., a Novel Actinobacterium Isolated from Mangrove Soil, Exerts a Cytotoxic Activity in vitro

Characteristic

1

2

3

4

Temperature for growth (°C)

14–37

20–37a

16–45b

16–42c

pH range for growth

4.5–9.5

6–8b

5–9b

5.5–8c

NaCl tolerance (%)

7

4b

3b

3c

Nitrate reduced to nitrite

+

Gelatin liquefaction

+

+

+

Glucose fermentation

w

+

w

Enzyme activity

Alkaline phosphatase

+

+

w

+

α-chymotrypsin

w

+

Esterase(C4)

w

Esterase lipase(C8)

w

w

α-galactosidase

w

β-glucosidase

w

+

w

w

Lipase(C14)

+

Naphthol AS-BI phosphohydrolase

w

N-acetyl-β-glucosaminidase

w

+

Urease

w

w

w

Valin arylamidase

w

w

Hydrolysis of:

Starch

+

+

w

Tween 20

+

+

Tween 60

+

w

+

+

Tween 80

+

Esculin

+

+

+

w

Utilization of:

N-Acetyl-glucosamine

w

+

w

w

D-Mannitol

w

+

w

Potassium gluconate

+

+

w

+

Malic acid

w

+

+

Major Polar Lipids

PE, DPG

PE, DPG, PIM, PSc

PE, DPG, PI, PIMb

PE, DPG, PIc

Major menaquinones

MK-10(H6)

MK-10(H6), MK-10(H4), MK-9(H4)d

MK-10(H6), MK-9(H6)b

MK-10(H6), MK-9(H6), MK-9(H4)c

DNA G+C content (mol%)

73.2

71.0a

71.7b

71.2c

  1. All data are obtained from this study unless otherwise indicated. +, Positive; −, negative; w, weakly positive.
  2. a:79. b:80. c21. d:16.