Figure 5

PD-L1 expression was enhanced by TGF-β-induced EMT. (A) YD-10B and HSC-4 cells were treated with the indicated concentrations of TGF-β1 (0.1, 0.5, and 1 ng/mL) for 2 days. (B) YD-10B and HSC-4 cells were incubated with medium containing 1 ng of TGF-β1 (TGF-β1 +) for 3 days, and then further incubated with medium without TGF-β1 (TFG-β1 –) for 5 days. (C) YD-10B and HSC-4 cells were treated with TGF-β1 (1 ng/mL) or TGF-β1 (1 ng/mL) plus SB 42152 (10 μM) for 2 days. (A–C) The levels of E-cadherin, vimentin, and PD-L1 were compared by western blotting (top) or real-time PCR (bottom). The real-time PCR data are presented as relative values normalized to those of the internal control (GAPDH). Full length uncropped western blotting images were shown in Supplementary Fig. S1.