Figure 8

Expression level of significant genes through quantitative real-time PCR analysis and strong associations between NRS, osmotic pressure, CCL5, and OPRL1. (A) The relative expression level of CCL5. (B) The relative expression level of OPRL1. (C) The relative expression level of SST. (D) The relative expression level of CXCL13. (E) The NRS was negatively associated with osmotic pressure. (F) There is a positive association between NRS and the relative expression of CCL5. (G) The NRS was positively associated with the relative expression of OPRL1. (H) There was also a positive association between the relative expression of OPRL1 and the relative expression of CCL5. (I) Osmotic pressure was negatively associated with the relative expression of CCL5. (J) Osmotic pressure was negatively associated with the relative expression of OPRL1. (K) The heatmap shows the strong associations between the NRS, osmotic pressure, CCL5, OPRL1, SST, and CXCL13 through Spearman correlation analysis. (L) The receiver operator characteristic curve indicates that the expression level of CCL5 could predict NRS sensitively and specifically. (M) The receiver operator characteristic curve indicates that the expression level of OPRL1 could also predict NRS sensitively and specifically.