Table 4 Validation of predicted points from the selected quadratic model.
Media composition and incubation conditions | Organisms | ||
|---|---|---|---|
B. safensis M35 | B. altitudinis J208 | ||
Media components (g/100 ml) | BHM | 0.327 | 0.327 |
Yeast Extract | 0.025 | 0.025 | |
Peptone | 0.075 | 0.075 | |
Inducer substrate (g/100 ml) | WB | 1.57 | 1.90 |
CP | 1.26 | 0.62 | |
Physical parameters for incubation | Initial pH | Default (5.8) | Default (6.2) |
Temperature (°C) | 37 | 37 | |
Agitation rate (rpm) | 160 | 160 | |
Inoculum (%v/v) | 0.5 | 0.5 | |
Response 1 Xylanase | 95% PI Low | 13.38 | 12.49 |
Prediction | 14.77 | 13.94 | |
95% PI High | 16.16 | 15.39 | |
Observed | 15.01 | 14.27 | |
Response 2 Pectinase | 95% PI Low | 324.44 | 376.16 |
Prediction | 371.54 | 601.01 | |
95% PI High | 418.64 | 823.84 | |
Observed | 411.58 | 728.74 | |
