Figure 3 | Scientific Reports

Figure 3

From: Immunization with DNA prime-subunit protein boost strategy based on influenza H9N2 virus conserved matrix protein M1 and its epitope screening

Figure 3

Mice primed with 100 μg DNA-boosted with 100 μg M1 protein generated more M1 specific IFN-γ producing T cells compared with mice immunized alone. Splenocytes harvested from mice 14 days after vaccination were stimulated with 50 μg/ml M1 pools for 22-24 h and scored in ELISPOT assays for IFN-γ producing cells. The values represent the averages of quadruplicate wells of 3 mice, and are expressed as means ± SD. The results were expressed as the number of SFC per 106 input cells. *Significant differences between the treatment groups and the control group (p < 0.05).

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