Figure 6
Peptide activity of M1 T cell epitope of H9N2 avian influenza virus. Two weeks after the DNA primary - 100 μg M1 intranasal boost immune strategy, the spleen lymphocytes were isolated, 2 × 105 responding cells were incubated in 96-well PVDF plates coated with anti-IFN-γ monoclonal antibody. According to a 5 × 5 matrix, the single peptide of the intersection of two positive peptide pools may be the M1 specific T cell epitope peptide, the preliminarily determined positive single peptides were respectively used as ELISPOT reaction stimulus (50 μg/ml peptide), then to calculate the specific spots after 20-24 hrs. The peptide with spots >10 was considered positive peptides. The values represent the averages of quadruplicate wells of 3 mice, and are expressed as means ± SD. The results were expressed as the number of SFC per 106 input cells.
