Table 3 Primers for real-time PCR.
Primer Name | Sequence | Product Length (bp) | TM (°C) |
|---|---|---|---|
s1GAPDHmrna | 5′-CATGACAACTTTGGTATCGTGGA-3′ | 381 | 62 |
as1GAPDHmrna | 5′-GTGGGTGTCGCTGTTGAAGTC-3′ | ||
s1ODC1mrna | 5′-CCGCTCGAGCGGATAAGTAGGGAGCGGCGTG-3′ | 276 | 54 |
as1ODC1mrna | 5′-ATCATGGCGACCCTACTCTTAC-3′ | ||
s1LINEmRNA | 5′-TGGAATAGGTGTGGTGTGGTGCT-3′ | 246 | 61 |
as1LINEmRNA | 5′-TCACTCAGGACAGCCCAGACG-3' | ||
sp21mrna | 5′-GGAAGACCATGTGGACCTGT-3′ | 146 | 55 |
asp21mrna | 5′-GGCGTTTGGAGTGGTAGAAA-3′ |
