Figure 5

Expression of RNA-i against seven distinct GPCRs blocked the increase in MIGSC in response to mating. (a–c) Bar graphs showing MIGSC. Blue: non-mated condition, red: mated condition, ***P-value <0.001, numbers of GSCs as indicated, genotypes as indicated. (a) Control males have significantly higher MIsGSC than their non-mated siblings. (b,c) Mated (b) GPCR-i/NG4-1 and (c) GPCR-i/NG4-2 males did not have significantly higher MIsGSC compared to their non-mated siblings.