Table 2 MIGSC from select RNA-i-lines directed against GPCRs.

From: G-protein signaling is required for increasing germline stem cell division frequency in response to mating in Drosophila males

GPCR

BL #

Single

Mated

Diff.

UAS-5-HT2A-i

31882

25/490 = 5.1

36/465 = 7.3

2.2

 

56870

19/582 = 3.3

32/553 = 5.8

2.5

UAS-5-HT2B-i

60488

6/261 = 2.3

21/272 = 7.7

5.4

 

25874

4/228 = 1.7

34/236 = 14.4

12.7

UAS-Ado-R-i

27536

11/276 = 4.0

20/209 = 9.6

5.6

UAS-AKHR-i

29577

23/492 = 4.7

54/574 = 9.4

4.7

UAS-AR-2-i

25935

13/363 = 3.6

25/336 = 7.4

3.6

UAS-CG13229-i

29519

9/285 = 3.2

33/297 = 11.1

7.9

UAS-CG14539-i

25855

21/318 = 6.6

31/307 = 10.1

3.5

UAS-CG15556-i

44574

28/425 = 6.6

43/401 = 10.7

4.1

UAS-CG15744-i

28516

18/279 = 6.4

27/252 = 10.7

4.3

 

42497

23/323 = 7.1

36/236 = 11.0

3.9

UAS-CG30106-i

27669

10/244 = 4.1

34/273 = 12.4

8.3

UAS-CG33639-i

28614

32/300 = 10.7

47/371 = 12.7

2.0

UAS-CCHaR1-i*

51168

27/407 = 6.6

27/323 = 8.4

1.8

UAS-Cry-i

43217

43/389 = 11.0

75/521 = 14.4

3.4

UAS-CrzR-i

52751

14/337 = 4.2

31/333 = 9.3

5.1

UAS-Dop1R1-i

62193

12/352 = 3.4

25/308 = 8.1

4.6

 

55239

11/300 = 3.7

44/267 = 16.5

12.8

UAS-GABA BR2-i

50608

6/176 = 3.4

35/207 = 16.9

13.5

 

27699

7/291 = 2.4

18/282 = 6.4

4.0

UAS-GABA BR3-i

42725

10/190 = 5.3

28/243 = 11.5

6.2

UAS-Moody-i

36821

5/301 = 1.7

16/234 = 6.8

5.1

UAS-Mth-l1-i

41930

11/279 = 4.0

42/300 = 14.0

10

UAS-Mth-l3-i

41877

54/817 = 6.6

81/850 = 11.8

5.2

 

36822

19/231 = 8.2

39/241 = 16.2

8.0

UAS-Mth-l8-i

36886

48/933 = 5.1

70/915 = 7.6

2.5

UAS-Mth-l9-i

51695

61/985 = 6.2

82/910 = 9.0

2.8

UAS-Mth-l15-i

28017

14/349 = 4.0

25/337 = 7.1

3.1

UAS-PK1R-i

27539

28/478 = 5.9

37/375 = 9.9

4.0

UAS-Smo-i

27037

12/263 = 4.6

33/288 = 11.4

6.8

 

43134

19/274 = 7.0

15/147 = 10.2

3.2

UAS-Tre1-i

34956

5/234 = 2.1

28/252 = 11.1

9.0

UAS-TKR86D-i*

31884

31/564 = 5.5

28/394 = 7.1

1.6

UAS-TKR99D-i

55732

30/506 = 5.9

49/467 = 10.5

4.6

 

27513

4/240 = 1.7

30/294 = 10.2

8.5

  1. UAS-driven expression of RNA-i for the listed GPCRs via NG4-1 did not block the increase in MIGSC in response to mating. BL #: Bloomington stock number, Single and Mated: number of pHH3-positive GSCs/total number of GSCs = MIGSC, Diff: MIGSC of mated males minus MIGSC of non-mated males. GPCRs marked by asterisks were excluded from further studies because their siblings outcrossed to wt did not show a stronger response to mating than the experimental (GPCR-i/NG4-1) flies.
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