Table 3 Experimental binding constants determined in solution.
Ligand | Dissociation constants (KD µM) | AgamOBP1_D78S | AgamOBP1_S82P | AgamOBP1_K93H | |
|---|---|---|---|---|---|
WT_AgamOBP1 | AgamOBP1_D78N | ||||
1-NPN | 0.49 ± 0.1 | 1.9 ± 0.5 | 2.03 ± 0.13 | 0.22 ± 0.1 | 0.83 ± 0.24 |
Atropine | 4.81 ± 1.72 | NA | 1.84 ± 1.03 | 0.34 ± 0.002 | NA |
Cocaine HCl | 11.57 ± 0.22 | 3.67 ± 0.35 | NA | 0.48 ± 0.14 | NA |
THC | 3.59 ± 0.66 | 2.19 ± 0.36 | 3.94 ± 0.73 | 0.28 ± 0.07 | 1.17 ± 0.05 |
Cannabinol | 0.26 ±0.08 | ND | ND | 0.06 ± 0.02 | 0. 22 ± 0.08 |
MDMA(Ecstasy) | 9.24 ± 0.48 | 5.19 ± 0.95 | 5.86 ± 1.15 | 0.08 ± 0.01 | 0.23 ± 0.02 |
Ephedrine | NA | NA | NA | 0.88 ± 0.17 | NA |
Heroin HCl | NA | 1.86 ± 0.58 | 4.39 ± 0.72 | 0.27 ± 0.17 | 0.47 ± 0.21 |
Codeine | NA | NA | 7.51 ± 1.47 | 0.65 ± 0.27 | 2.48 ± 0.19 |
NA = No binding observed. | |||||
ND = Was not determined. | |||||
