Figure 6
Overall and regional immunohistochemistry for HT, HT+V and HT+M groups for IBA1 ramification index, GFAP luminosity, Cleaved Caspase 3 (CC3) and OLIG2. For IBA1 ramification index, there was no difference in overall ramification index in the HT+M versus HT+V or HT alone (A). On regional analysis the caudate showed an increase in IBA1 ramification index in the HT+V versus HT (E). Representative photomicrographs are shown for HT (I), HT+V (M) and HT+M (Q). There was no difference in overall astrogliosis (GFAP) (B), but localized increases in astrogliosis in the cCTX, IC and caudate as shown (F). Representative GFAP photomicrographs are shown for HT (J), HT+V (M) and HT+M (R). There was an overall increase in CC3 in the HT+M versus HT and HT+M versus HT+V (C). Localized differences with increased CC3 mainly in the HT+M group were compared to both HT+V and HT alone were seen in cCTX, sCTX, pvWM, caudate, putamen, thalamus (G). Representative CC3 photomicrographs are shown for HT (K), HT+V (O) and HT+M (S). There was an overall increase in OLIG2 counts in the HT+M and HT+V compared to HT (D). Localised differences were seen with increased OLIG2 mainly in the IC and pvWM (H). Representative OLIG2 photomicrographs are shown for HT (L), HT+V (P) and HT+M (T). Cingulate cortex = cCTX; Sensorimotor cortex = sCTX; Hippocampus = HIP; Periventricular white matter = PvWM; Internal capsule = IC; Caudate = CAUD; Putamen = PTMN; Thalamus = THAL. Error bars represent standard error ***p < 0.001, **p < 0.01, *p < 0.05.
