Figure 1

The time-course of the singlet oxygen phosphorescence signal for AsLOV2 C450A (A) and AsLOV2 wt (B) at different irradiation levels. The six curves were fitted in a global fitting procedure; the lines represent the fitted results. Insets: The decay of the FMN triplet state absorbance at 633 nm following the excitation laser pulse as measured for AsLOV2 C450A and AsLOV2 wt. Black and magenta points correspond to low and high irradiation, respectively.