Figure 5
Fluorescence emission spectra of FMN and intrinsic tryptophan in AsLOV2 wt and AsLOV C450A. The fluorescence of FMN in AsLOV2 wt (A) and in AsLOV2 C450A (B) before (black line) and after (red dots) illumination and the fluorescence of free FMN (green). The blue line shows the fit to FMN fluorescence after the illumination. The fits (blue line) consist of two fractions of fluorescence: protein-bound and free FMN. Insets: Intrinsic tryptophan fluorescence of the corresponding forms of AsLOV2 before (black line) and after illumination (red line). The concentrations of proteins and the free FMN were 10 µM in 20 mM Tris-HCl, pH 7.8.
