Figure 8

List of oxidation products and their extent of oxidation (intensity of oxidatively modified peptide) in all studied protein forms – wild-type (wt) and C450A (mut) before (N) and after (I) irradiation. Column Mod shows the elemental composition of the modification (oxidation: +O1, +O2 or +O3; Trp to kynurenin: -C1+O1; Pro to pyrrolidone: -C1O1). When it was not possible to clearly assign oxidation to one specific residue, two possibly affected residues are listed in the first column. The last column highlights the fold change in oxidation between C450A and wt forms. In case of C450 oxidation (marked in the last column with an asterisk) such comparisons were not possible due to generation of different peptides upon oxidation and due to mutation. The intensity of oxidized form was indirectly deduced from intensity decrease of the intact, unoxidized Asp-N generated peptide.