Table 1 Relationship between frozen-thawed sperm DFI and pup birth rates of embryos derived by IVF or ICSI using frozen-thawed sperm.

From: DNA fragmentation index (DFI) as a measure of sperm quality and fertility in mice

 

Sperm Count (×106)

Total Motility (%)

Prog. Motility (%)

Normal Heads (%)

Normal Tails (%)

Sperm DFI (%)

2-cell Rate (%)

Embryos transferred

Pup Rate (%)

IVF no pups (n = 5)

20.3 ± 10.2

45.2 ± 9.3

19.0 ± 6.2

70.6 ± 17.4

68.8 ± 11.2

62.7 ± 7.2*

14.4 ± 9.4#

206

0&

IVF Ctrl (n = 7)

19.8 ± 3.8

54.4 ± 16.4

29.7 ± 12.0

74.7 ± 8.4

78.9 ± 3.8

20.4 ± 7.9*

32.7 ± 4.9#

380

36.4 ± 12.2&

ICSI no pups (n = 5)

7.9 ± 3.0§

29.6 ± 24.2

3.6 ± 1.9@

31.2 ± 16.6Δ

50.2 ± 31.7

73.3 ± 8.1&

79.6 ± 11.4

188

0®

ICSI Ctrl (n = 6)

22.5 ± 7.2§

42.2 ± 12.3

21.2 ± 9.1@

73.2 ± 6.9Δ

71.0 ± 6.6

28.1 ± 10.7&

85.8 ± 5.9

240

23.4 ± 6.0®

  1. Note: The data with the same symbols in the same column are significantly different (P < 0.05). The total and progressive motilities were obtained before sperm freezing.