Figure 4
(a) Quantitative imaging analysis of ROS detected by DHE in live MCF-7 cells. Fluorescence intensity was assessed using ImageJ 1.47 v software (http://imagej.nih.gov/ij). Experiments are in triplicate and presented data are the mean ± s.e.m. **P < 0.01 (P = 0.0080) (student’s t-test), (b) Agrose Gel Electrophoresis demonstrating DNA fragmentation. MCF-7 cells treated with 15 µg/mL 5-FU+DOX@CMCS, which induces DNA fragmentation in 48 hours. Campothecin treated cells for 48 h served as the positive control (PC), (c) Comparison of relative intensity calculated from the different lanes agarose gel using ImageJ 1.47 v software (http://imagej.nih.gov/ij). Experiments are in triplicate and presented data are the mean ± s.e.m. ****P < 0.0001 (student’s t-test), (d) %Hemolysis exhibited by the various prepared samples. Values are expressed as Mean ± S.D. of 3 independent experiments. ****P < 0.0001, ***P = 0.0005, **P = 0.0027 (One way anova).
