Table 2 The expression pattern of 22 DEGs validated through qRT-PCR and KEGG pathway analysis results in test hybrid, ↓ denotes down-regulation and ↑ denotes up-regulation.

From: Genome-wide transcriptome profile of rice hybrids with and without Oryza rufipogon introgression reveals candidate genes for yield

S. No

Gene name

Description

Microarray

qRT-PCR

KEGG pathway analysis

Leaf

Panicle

Leaf

Panicle

1

Os02t0559800-01

E3 ubiquitin ligase EL5

↓

↑

↓

↑

—

2

Os02t0624300-01

Similar to Y19 protein

↓

↑

↓

↑

—

3

Os08t0482600-01

Cupredoxin domain containing protein.

Ā 

↑

Ā 

↑

Ā 

4

Os08t0473900-03

Similar to Alpha-amylase isozyme 3D

Ā 

↑

Ā 

↑

Starch degradation

5

Os08t0473900-01

Alpha-amylase isozyme 3D precursor

Ā 

↑

Ā 

↑

Starch degradation

6

Os08t0466200-01

Similar to KUP-related potassium transporter.

Ā 

↑

Ā 

↑

Ā 

7

Os02t0569900-01

Cytochrome P450 family protein-1500bp

↓

Ā 

↓

Ā Ā 

8

Os02t0626400-01

Phenylalanine ammonia-lyase

↓

Ā 

↓

Ā 

Suberin biosynthesis, phenyl propanoid bio synthesis and initial reactions, salycilate biosynthesis

9

Os03t0753100-01

MADS-box transcription factor, Inflorescence and spikelet development

↑

Ā 

↑

Ā Ā 

10

Os10t0531400-01

Glutathione S-transferase GST 30

↓

Ā 

↓

Ā 

Glutathion mediated detoxification

11

Os03t0401300-01

Sucrose synthase 2

↓

Ā 

↓

Ā 

Sucrose degradation to ethanol and lactate (anaerobic), galactose degradation II, starch and sucrose metabolism, sucrose degradation III, sucrose biosynthesis

12

Os01t0348900-01

SalT gene product (Salt-induced protein).

Ā 

↑

Ā 

↑

Ā 

13

Os01t0314800-01

Late embryogenesis abundant protein 3 family protein

Ā 

↑

Ā 

↑

Ā 

14

Os03t0815100-01

Similar to OsNAC6 protein

Ā 

↑

Ā 

↑

Ā 

15

Os04t0180400-01

Similar to Cytochrome P450 99A2

↓

↑

↓

↑

Momilactone biosynthesis, nicotine degradation II, nicotine degradation III, bupropion degradation, acetone degradation to methyl glyoxal

16

Os09t0334500-01

WRKY transcription factor 74.

Ā 

↑

Ā 

↑

Ā 

17

Os12t0559200-01

Lipoxygenase

↓

Ā 

↓

Ā 

Biosynthesis of plant harmones, JA biosynthesis, alpha-lenolenic acid metabolism, 13-LOX and 13 HPL pathway, divinyl ether biosynthesis II, lenoleic acid metabolism

18

Os08t0498100-01

Similar to Caffeoyl-CoA O-methyltransferase 2

↓

Ā 

↓

Ā 

Phenyl propanoid biosynthesis, suberin biosynthesis

19

Os02t0626100-01

Similar to Phenylalanine ammonia-lyase.

↓

Ā 

↓

Ā 

Phenyl propanoid biosynthesis, suberin biosynthesis, phenyl alanine metabolism, phenyl propanoid biosynthesis initial reactions, salicylate biosynthesis, Tropane, piperidine and pyridine alkaloid biosynthesis, nitrogen metabolism

20

Os08t0448000-01

Similar to 4-coumarate-CoA ligase 1

Ā 

↑

Ā 

↑

Biosynthesis of phenyl propanoids, Biosynthesis of alkaloids derived from shikimate pathway, phenyl propanoid biosynthesis, metheonine biosynthesis II, Ubiquinone and other terpenoid-quinone biosynthesis, threonine biosynthesis from homoserine

21

Os10t0465700-01

Similar to Beta-amylase PCT-BMYI

↓

↑

↓

↑

starch degradation

22

Os02t0738200-01

Zinc finger, RING/FYVE/PHD-type domain containing protein.

↓

Ā 

↓

Ā Ā