Figure 3
Duplex real-time RPA assay. (a) Assay using a mixture of primer B. caballi-bc48-F1/R2 and T. equi-ema-F1/R1 for pUC57-bc48 plasmid DNA (lines b, c) and pUC57-ema plasmid DNA (lines a, d). (b) Assay for T. equi genomic DNA using primer T. equi-18S-F3/R2 (solid line) and T. equi-ema-F1/R1 (short dashed line). (c) T. equi-18S-F3/R2/B. caballi-18S-P for pUC57-Te18S plasmid DNA (line a) and pUC57-Bc18S plasmid DNA (line b); B. caballi-18S-F3/R3/T.equi-18S-P for pUC57-Bc18S plasmid DNA (line c) and pUC57-Te18S plasmid DNA (line d). (d) T. equi-18S-F2/R2/B.caballi-18S-P for pUC57-Te18S plasmid DNA (line a) and pUC57-Bc18S plasmid DNA (line b); B. caballi-18S-F2/R2/T. equi-18S-P for pUC57-Te18S plasmid DNA (line c) and pUC57-Bc18S plasmid DNA (line d). (e) Mixture of T. equi-18S-F3/R2 and B. caballi-18S-F3/R2 for pUC57-Te18S plasmid DNA (lines a, a’) or pUC57-Bc18S plasmid DNA (lines b, b’). (f) Mixture of T. equi-18S-F2/R2 and B. caballi-Bc48-F1/R2 for B. caballi genomic DNA (lines, a, a’), T. equi genomic DNA (lines, b, b’) and water (lines, c, c’).
