Figure 4
Sensitivity of real-time RPA assay. (a) pUC57-Bc48 plasmid DNA with primer B. caballi-bc48-F1/R2. (b) pUC57-ema plasmid DNA with primer T. equi-ema-F1/R1; lines a-h, from 0.2 ng to 0.02 fg. (c) pUC57-Bc18S plasmid DNA with primer B. caballi-Bc18S-F3/R2. (d) pUC57-Te18S plasmid DNA with primer T. equi-18S-F3/R2/; lines a-g, from 2 ng to 2 fg. Line h, water. (e) Real-time PCR assay for serially-diluted pUC57-Bc18S plasmid DNA; (f) Real-time PCR assay for serially-diluted pUC57-ema plasmid DNA; lines a–g, from 2 ng to 2 fg. Line h, water.
