Figure 5
Fluorescence curves of horse blood assay using real-time PCR and real-time RPA methods. (a) Real-time RPA assay for B. caballi genomic DNA using primer B. caballi-bc48-F1/R2/bc48-P (solid lines, three samples) and B. caballi-18S-F3/R2/bc-18S-P (short dashed lines, three samples). Line 11, a; Line 12, b; Line 13, c. (b) Real-time PCR assay for real samples containing B. caballi genomic DNA using Bc_18SF402/ c_18SR4596/Bc_18SP. Lines 1–10, horse blood samples, numbered HQ1–10. (c) Real-time PCR assay for real samples containing T. equi genomic DNA using Te_EMA1-F/ Te_EMA1-R/Te_EMA1-P. Lines 14 and 15, horse blood samples, numbered DQ1 and DQ2. (d) Real-time RPA assay for real samples containing B. caballi genomic DNA using B. caballi-bc48-F1/R2/bc48-P. Lines 1–10, HQ 1–10. (e) Real-time RPA assay for real samples containing T. equi genomic DNA using T. equi-18S-F2/R2/T. equi-18S-P. Line 14, DQ1; line 15, DQ2; lines 1–3, HQ 1–3. (f) Real-time fluorescence curves of a duplex assay for B. caballi genomic DNA (line 1, HQ1; 2, HQ2; 3, HQ-3; 4, HQ-4), T. equi genomic DNA (line 14, DQ1; 15, DQ2), trypanosoma evansi (line 16), trypanosome brucei (line 17), toxoplasma gondii (line 18), plasmodium falciparum (line 19), theileria hirci (line 20), theileria sinensis (line 21), trichinella spiralis inorganic (line 22). Solid lines indicated the FAM signal generated by the bc48 gene of B. caballi, and short dashed lines indicated the ROX signal generated by the 18S gene of T. equi.
