Figure 6

Repeatability of the RPA assay. (a) The real-time fluorescence curves of the RPA assay for 0.02 ng (solid line, four samples) and 20 fg (short dashed lines, four samples) of pUC57-bc48 plasmid DNA. (b) The onset time of real-time amplification of four assays. The colour of the columns corresponds to the colour of the lines in part (a).