Table 3 Sequences of primers and probes used in the exo RPA assays for the 18S rRNA gene of Theileria equi and Babesia caballi.

From: Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis

Name

Sequences 5′-3'

B. caballi-18S-F1

ACAGCGACAAGCTGTAGGGAAGTTTAAGGC

B. caballi-18S-F2

CTTCCCTTTTTTTGTTTGGGTTTGCTTCTTAGAG

B. caballi-18S-F3

GGGTTTGCTTCTTAGAGGGACTTTACAGCGACAAG

B. caballi-18S-R1

CCCACACCTTTCGGAGCAGGAAAAACTTAGTGA

B. caballi-18S-R2

CACCTTTCGG AGCAGGAAAAACTTAGTGAATGCA

B. caballi-18S-R3

AGGCAAAACCGACGAATCGGAAAAGCCACGGTCCG

B. caballi-18S-P

ACAAGCTGTAGGGAAGTTTAAGGCAATAACAGG(FAM-dT)(THF)(BHQ1-dT)GTGATGCCCTTAGA-C3 spacer

T. equi-18S-F1

ATAGGGTGTGAGACTTGGTTTCATTTCCGCTTC

T. equi-18S-F2

GTGAGACTTGGTTTCATTTCCGCTTCTTAGAGGG

T. equi-18S-F3

GGTGTGAGACTTGGTTTCATTTCCGCTTCTTAGA

T. equi-18S-R1

ATTACCCAAGCCTCTCAGCCAAGGATACACTC

T. equi-18S-R2

AGCCTCTCAGCCAAGGATACACTCAGTGAATGC

T. equi-18S-P

AGGGACTTTGCGGTCATAAATCGCAAGGAAG(ROX-dT)(THF)(BHQ1-dT)AAGGCAATAACAGG-C3 spacer

  1. Note: FAM-dT and ROX-dT: thymidine nucleotide carrying Fluorochrome; THF: tetrahydrofuran spacer; BHQ1-dT: thymidine nucleotide carrying Black Hole quencher.
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