Table 4 Nucleotide sequences of primers and probes used in the duplex qPCR assay.

From: Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis

Name

Sequences 5′-3′

Bc_18SF402

GTAATTGGAATGATGGCGACTTAA

Bc_18SR4596

CGCTATTGGAGCTGGAATTACC

Bc_18SP

VIC-CCTCGCCAGAGTAA-MGB-NFQ

Te_EMA1-F

CTGACTACAAGGTYGTATAC

Te_EMA1-R

TGTCGTCACTT AGTAAAATAGA

Te_EMA1-P

6-FAM-TTCTCCGTCTATGGCGCA-MGB-NFQ

  1. Abbreviations: MGB, minor groove binder; NFQ, non-fluorescent quencher
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