Figure 3 | Scientific Reports

Figure 3

From: Angiotensin II represses Npr1 expression and receptor function by recruitment of transcription factors CREB and HSF-4a and activation of HDACs

Figure 3

Effect of ANG II Type 1 and Type 2 receptor antagonists on ANG II-mediated transcriptional repression of Npr1 gene promoter in the ∆R1 and ∆R5 constructs. (A) Schematic representation of the Npr1 promoter constructs ∆R1 (−1182 to −1127 bp) and ∆R5 (−984 to −914 bp). MMCs were transiently transfected with 1 µg of either ∆R1 construct (B,C) or ∆R5 construct (D,E) and 0.3 µg of pRL-TK. After 24 h, the cells were treated with either 100 nM PD 123319 (B,D) or 100 nM candesartan (C,E), followed by incubation with 10 nM ANG II for 16 h. Normalized luciferase activity is shown as a percentage of the activity of an untreated group. The results are expressed as mean + SE from 7–8 independent experiments. **p < 0.01.

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