Figure 4 | Scientific Reports

Figure 4

From: Angiotensin II represses Npr1 expression and receptor function by recruitment of transcription factors CREB and HSF-4a and activation of HDACs

Figure 4

Effect of protein kinase inhibitors on ANG II-mediated transcriptional repression of Npr1 gene promoter in ∆R1 and ∆R5 constructs. MMCs were transiently transfected with 1 µg of ∆R1 or ∆R5 construct and 0.3 µg of pRL-TK. After 24 h, cells were treated with 100 nM H-89 dihydrochloride (A,D), 100 nM wortmannin (B,E), or 100 nM genistein (C,F) and were incubated further with 10 nM ANG II for 16 h. Normalized luciferase activity is shown as a percentage of the activity of untreated groups. (G) Schematic representation of the effect of protein kinase inhibitors on Npr1 gene transcription. The results are expressed as mean + SE from 7–8 independent experiments. **p < 0.01.

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