Figure 4

Biocompatibility of 3D scaffold and illustration of the fabrication 3D scaffold using a magneto-acoustic field. (a) The effect of medium after 4-days incubation with CP-based 3D scaffold on the cell viability (Alamar Blue assay, 72 h). (b) SHED tissue spheroid from 27000 cells in Spheroid microplate (bar 100 μm). (c) CP-based 3D scaffold immediately after fabrication (bar 2000 μm). (d) Schematic showing of SHED cells (green) attachment and migration on the surface and inside the 3D scaffold. (e) CP-based 3D scaffold incubated with SHED spheroids (0 h). (f) CP-based 3D scaffold incubated with SHED spheroids within 7 days. (g) Fluorescence and light microscopy images of 3D scaffold incubated with SHED spheroids (green) within 7 days. (h) SEM image of 3D scaffold with SHED spheroids on the surface after 7 days incubation (bar 50, 100 μm). (i) The schematic representation of the fabrication of a three-dimensional scaffold using a magnetoacoustic field, including the assembly and recrystallization of the scaffold.