Figure 4

Characterization of Corneal Puncture Process. (A) Quantification of solenoid device instantaneous velocity vs. time immediately after firing as determined from high speed video footage. Velocity was quantified for 3 technical replicates with a 4.2 mm and 0.45 mm diameter object attached. Vertical dashed line represents the travel distance when the puncture object would make contact with the eye. Note the solenoid device contains a retraction spring which results in deceleration. Quantification of (B) force and (C) kinetic energy for 2 CP object sizes at the travel distance when contact with the eye would occur (n = 3 technical replicates). No significant differences between the object sizes were found. (D) Pressure vs. time traces for the catheter pressure transducer and in-line transducer connected by anterior needle cannulation following contact with the 2.4 mm diameter CP object at 0.02 seconds. (E) Quantification of the maximum IOP recorded during the CP injury process for the in-line transducer and catheter transducer (n = 4 biological replicates). (F) Quantification of the difference between the times (milliseconds) at which maximum IOP was measured by the in-line and catheter transducers (n = 4 biological replicates). Statistical differences between the two groups was determined by Student’s t-test, where p < 0.05 denotes statistical significance.