Figure 5

Compound responses in a panel of primary ADPKD and normal human kidney (NHK) epithelial cells. (A) Pairwise analysis of similarity of compound response between kidney cell preparations. Cell pairs displaying similar responses (p-val > 0.05) are colored yellow and those displaying different responses (p-val < 0.05) are colored pink. ADPKD cells are labeled in black and NHK in red. Left: CTG readout; Right: GF-AFC readout. (B) Mean ΔAUC ± SD of compounds with differential response in ADPKD vs. NHK cell pairs. Only compounds with differential responses in more than 6 pairs (with at least 2 different ADPKD and 3 different NHK cell isolates) are shown. The size of the node corresponds to the number of cell pairs. (C) Dose response curves of antimetabolites displaying differential response in pairwise analysis of ADPKD (black) and NHK (red) primary cells in the CTG readout. Responses obtained with GF-AFC reagent are shown in Supplementary Fig. 9. Data is represented as Mean ± SD, n = 3.