Figure 4
Profiling of human, rat, and mouse serum sEV miRNAs. (A) Correlation between normalized miRNAs read counts (Log2TPM) between the biological replications from each group. (B) Correlation between normalized read counts (Log2TPM) obtained by small RNA-seq and adjusted q-RT-PCR CT values for 6 miRNAs (miR-125a-5p, miR-125b-5p, miR-191-5p, miR-27b-3p, miR-486-5p and miR-99a-5p). q-RT-PCR for miRNAs were performed with the same batch of RNAs that prepared for sequencing. The relative expression of the selected miRNAs as determined by qPCR were expressed as (40-Ct) miRNA. TPM, transcripts per million reads; h, human; r, rat; m, mouse; e, serum small extracellular vesicle; d, small extracellular vesicle depleted serum.
