Figure 3
Seahorse analysis reveals decreased mitochondrial respiration in the cortex and hippocampus, but not in the EC, of aged male APOE4 mice. Seahorse analysis was performed in order to analyze the effects of differential APOE isoform expression on mitochondrial respiration in mitochondria that were isolated from the cortex (Ctx), hippocampus (Hip) and EC of 20-month-old APOE mice (2 APOE4/4 males, tissues pooled vs. 2 APOE3/3 males, tissues pooled). (A) The oxygen consumption rate (OCR) from each region shows decreased mitochondrial respiration in the Ctx and Hip, but not the EC of the aged APOE4/4 mice. (B,C) Bar graphs showing the average OCR from (B) State 3 and for (C) the Respiration Control Ratio (RCR; state 3 u/state 4o) in each region of the APOE4/4 mice, as a percentage of the APOE3/3 OCR from the equivalent tissues. The dotted blue line represents the normalized levels in the APOE3/3 tissues. (**denotes p < 0.01; ***denotes p < 0.001; ****denotes p < 0.0001).
