Table 3 Comparison of on-field detection protocols for CITES-listed shark species identification.
| Â | LAMP assay | Multiplex real-time PCR assaya | Genome skimming using MinION hand-held sequencerb | |
|---|---|---|---|---|
Cost per sample | USD$ 0.6 | USD$ 0.94 | USD$ 0.8 (Extraction only) | |
Equipment | Multi-block thermal cycler | Real-time thermal cycler | MinION sequencer | |
Time required | Number of sample | Â | Â | Â |
1 | 3 hours | 3 hours | 12–40 hours | |
10 | 3 hours | 3 hours | 12–40 hours | |
94 | 14 hours | 4 hours | 12–40 hours | |
Tested with processed shark fin | Yes | Yes | No | |
Bioinformatics | Simple | Simple | Need expertise | |
Coverage of CITES species | All 12 species | 9 out of 12 species | All 12 species | |
Additional genetic information | No | No | Yes | |
