Figure 4
Glycogen metabolism Pathway activation in KUS-treated retinal ganglion cells. Pathway analysis of genes which were upregulated (fold change > 2) or downregulated (fold change < 0.5) between groups were done using an analysis software, Gene Spring14 (TOMY Digital Biology, http://genespring-support.com/). Pathways that were common in NMDA-saline < non-treat, KUS121 > NMDA-saline and KUS187 > NMDA-saline included glycogen metabolism pathway. Genes included in the current analysis, which passed p < 0.01 with analysis of variance (255 genes), are shown in orange color boxes. The bar graph next to the genes in the orange color boxes show the normalized expression values (N: non-treat, S: NMDA-saline, 121: KUS121, 187: KUS187). Phkb was an only gene which passed the upregulated and downregulated criteria above; downregulated in NMDA-saline compared to non-treat and was upregulated in KUS121 and KUS187 compared to NMDA-saline. The gene is shown in red frame box. non treat: retinal ganglion cells of mice without NMDA injection nor KUS treatment, NMDA-saline: retinal ganglion cells of NMDA-injected mice administered vehicle, KUS121: retinal ganglion cells of NMDA-injected mice administered KUS121, KUS187: retinal ganglion cells of NMDA-injected mice administered KUS187. In fold change analysis, >2 indicates more than 2 folds while <0.5 indicates less than 0.5 fold. For pathway analysis, A < B means statistically significant pathways of genes that were downregulated in group A by less than 0.5 fold compared to group B, and A > B means statistically significant pathways of genes that were upregulated in group A by more than 2 folds compared to group B.
