Figure 3
Longitudinal assessment of tumour rejection using MSOT. (a,b) Merged MSOT images of the gradient signal indicating cellular (red colour-coding) overlaid onto the anatomical optoacoustic images. One representative animal of the therapy (a) and one of the control group (b) is shown. Animals were imaged every second day starting at day 3 after T cell transfer. (c) 3D maximum intensity projection rendering of the merged MSOT images for the whole tumour volume of the animal shown in (a). Scale bar (MSOT) = 2.5 mm. (d) Tumour volumes (in mm3, blue colour-coding) from animals of the therapy (left image) and the control group (right image). The gradient signal per tumour volume (in %) is illustrated in red. Values are given as boxplots (mean; whiskers: min to max). Day 3 was used as baseline. Statistically significant differences of the gradient signal per tumour volume within the therapy are depicted above the relative day (A,B,C). The statistically significant difference of the tumour volume within the control group is marked for day 9 (D). Statistically significant differences of the gradient signal per tumour volume between the two groups (therapy and control) are depicted in the therapy group for day 7 and day 9 (1,2) and for the tumour volume in the control group at day 9 (3). p ≤ 0.05.
