Figure 8
From: Immune-mediated febrile response in female rats: Role of central hypothalamic mediators
CRFI/II, μ, NK1 and RANK receptors are not expressed in hypothalamic astrocytes in LPS-treated rats. Representative confocal images of hypothalamic samples from sham-operated and ovariectomized (OVX) female rats, treated with LPS (50 µg/kg, i.p.) or saline (100×). All panels show merged images of blue (cell nuclei, DAPI), green (astrocytes, GFAP), and red (CRFI/II, μ, NK1 and RANK) channels. Panels A, E, I and M show immunoreactivity for CRFI/II, B, F, J and N for μ receptors, (C,G,K,O) for NK1. Arrows point to immunoreactivity signals of CRFI/II, μ, NK1 and RANK. Note absence of co-localization with astrocytes. High-magnification inserts (400×) show the lack of co-localization in greater detail.
