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Figure 2

From: Contribution of neuronal calcium sensor 1 (Ncs-1) to anxiolytic-like and social behavior mediated by valproate and Gsk3 inhibition

Figure 2

HDAC inhibitors do not induce Ncs-1 expression. (a) Time course and dose response of Ncs-1 protein expression in SAHA-treated PC12 cells (n = 4/group). (b) Representative western blot (left) and densitometry quantification (right) of Ncs-1 protein expression in frontal cortex or striatum of mice chronically injected with MS-275 (20 mg/kg i.p. daily for 21 days) or vehicle (n = 5/group). In all cases data are represented as mean ± SEM. The bands of interest were cropped from the same membrane. Western blot quantifications were normalized against β-tubulin and plotted relative to vehicle-treated samples, which was set to 1. Significant differences were determined by two-way ANOVA followed by Tukey’s multiple comparison tests (a) or Student’s t-tests (b). Asterisks (*) in the figures indicate the p-values for the post-hoc test and correspond to the following values: *p < 0.05; **p < 0.01; ***p < 0.001, based upon mean ± standard error of mean.

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