Figure 3
Electrophysiological profile of gyroxin_B1.3 (A), BjSP (B) and chymotrypsin (C), on hEAG1. Superimposed current traces before (black) and after application (purple, orange and brown traces) of the samples, at 5 µM. (D) Multiple alignment of the sequences of proteins tested on hEAG1. Universal Protein Resource accession code: A0A0S4FKT4 for collinein-1, B0FXM1 for gyroxin_B1.3, Q5W959 for BjSP and Q7M3E1 for chymotrypsin. Marked light blue residues represent the common interaction sites between the ligands and the target, according to the proposed docking model. Marked red residues represent the common catalytic triad of serine proteases. The green box highlights the mutation made (His43 → Arg43) on the sequence of collinein-1, to turn it catalytically inactive (rCollinein-mut). The arrow indicates the Arg79 residue.
