Figure 2
From: Bacterioplankton reveal years-long retention of Atlantic deep-ocean water by the Tropic Seamount

The deep-water in situ incubator design. The incubator is a tethered water sampling rosette of twelve 20 L Niskin bottles with lids arranged into six sets of paired bottles. A set (a photo to the left, a scheme to the right) consists of two bottles connected at the top by a tubing line with an in-line propeller pump. The two tubing lines at the bottom are deployed closed and disengaged from the bottles. The bottom tubing line can be simultaneously opened and attached to the paired bottles using the two mechanical closures. Before deployment each tubing line is filled with water solution contained fixative or tracer. The incubator can be either operated via a seabird deck unit or pre-programmed for autonomous operation at a target depth.