Figure 2
From: Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients
Cell-bound IgE detection. (A) To confirm that all cell-bound IgE was dissociated from the cell membrane, anti-IgE PE-conjugated antibody was used for staining before or after lysis buffer treatment, flow cytometry was performed to analyze the IgE+ cell percentages in WBCs; (B) IgE+ cells in allergic patients and healthy people were evaluated before and after lysis buffer treatment; and C, we detected the dissociated cell-bound IgE and plasma IgE levels by ELISA.
