Figure 4
Cell cycle analysis after treatment with TA. 1 × 104 cells of (A) HROC173 and (B) HROC257 T0 M1 were seeded and after overnight incubation treated with increasing TA concentrations. Cell cycle phases as well as numbers of cells with Sub-G1 DNA were examined at 24 and 72 h after treatment. Residual cells were mainly arrested in G1 or G2/M-phase. Results show data of three independent experiments. Mean ± SD, *p < 0.05; **p < 0.01; one way ANOVA on ranks (Bonferroni’s Multiple Comparison Test). (C) Representative flow cytometry blots of HROC173 cells either being untreated (control, upper panel) or treated with TA (middle and lower panel) for 72 hours.
