Figure 5
Detection of senescence in HROC173 and HROC257 T0 M1 cells after incubation with 50 µM TA. 1 × 104 cells were seeded and after overnight incubation treated with 50 µM TA or PG for 5 days. Senescent cells were detected by blue color after beta-galactosidase assay. Treated cell show increased numbers of beta-galactosidase-positive cells. (A) Representative light microscopic images. Original magnification 10x. (B) X-Gal quantification. HPF – high power field; Mean ± SD, ***p < 0.0001; two-tailed t-test.
