Figure 5

Phase-contrast microscopic image (100×) (Nikon Ti Eclipse, Nikon, Japan) of actively proliferating buffalo mammary epithelial cells (BuMECs). The BuMECs were grown in DMEM supplemented with 10% FBS, 10 ng/ml EGF, 200 mM glutamine, 1 ug/ml hydrocortisone and 5 ug/ml Bovine insulin at 37 °C in a humidified atmosphere of 5% CO2. The BuMECs exhibit cobblestone appearance typical of epithelial morphology.