Figure 2 | Scientific Reports

Figure 2

From: The role of Ca2+/NFAT in Dysfunction and Inflammation of Human Coronary Endothelial Cells induced by Sera from patients with Kawasaki disease

Figure 2

Ca 2+/NFAT were activated in coronary artery endothelial cells induced by KD sera. (a,b) HCAECs were incubated with HC sera (pooled from 8 individuals), KD sera (pooled from 12 individuals) and febrile control (FC) sera (pooled from 10 individuals) for 6 hours. The cytoplasm proteins and nucleus proteins were extracted by Nuclear and Cytoplasmic Protein Extraction Kit. Levels of NFATc1 and NFATc3 proteins were measured using Western blotting (in a and b). The NFATc1, b-tubulin and Histone H3 blots in one group were cropped from different parts of the same gel. Full-length blots are presented in Supplementary Fig. S1. Data in (a,b) are mean ± SEM from 3 independent experiments involving different batches of cells but the s ame pooled HC or KD sera. *P < 0.05 and **P < 0.01 when compared with respective controls or between indicated groups.

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