Figure 4
CsA inhibited inflammation and attenuated dysfunction via Ca2+/NFAT. (a,b) HCAECs were pretreated with KD sera (pooled from 12 individuals) or HC sera (pooled from 8 individuals) for 6 hours and treated with or without CsA (500 ng/ml) for 2 h. VCAM-1, ICAM-1, TF, P-selectin, E-selectin, MCP-1 and IL8 mRNA and protein were detected and quantified. (b) The VCAM-1 and GAPDH blot in one group were cropped from different parts of the same gel. The NFATc1 and GAPDH blot in one group were cropped from different parts of the same gel. Full-length blots are presented in Supplementary Fig. S1. (c) HCAECs were pretreated with KD sera (pooled from 12 individuals) for 24 hours and treated with different concentration of CsA for 24 h. Cells proliferation were detected and quantified. (d) HCAECs were plated on Matrigel in the presence KD sera (pooled from 12 individuals) or co-cultured with KD sera and CsA. After 4 hours, cells were photographed and quantified. Data are mean ± SEM from 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 compared with the control or indicated group.
